Departmental Bulletin Paper イソスジエビのインスリン様造雄腺因子の血中動態解析に向けた高感度測定系の確立
Development of a Sensitive and Specific Enzyme-Linked Immunosorbent Assay (ELISA) for an Insulin-Like Androgenic Gland Factor of the Pacific Grass Shrimp Palaemon Pacificus

大平, 剛  ,  Ohira, Tsuyoshi  ,  埼玉, 瑞帆  ,  Saitama, Mizuho  ,  鈴木, 竜太  ,  Suzuki, Ryuta  ,  鶴岡, 慎哉  ,  Tsuruoka, Shinya  ,  片山, 秀和  ,  Katayama, Hidekazu  ,  泉, 進  ,  Izumi, Susumu

26pp.75 - 78 , 2015-06-30 , 神奈川大学総合理学研究所
The male secondary sex characteristics of crustaceans are controlled by an androgenicgland hormone (AGH), which is specifically synthesized in and secreted from the androgenic glands (AGs) attached to the male reproductive organ. AGH has been purified from the terrestrial isopod Armadillidium vulgare for the first time, and its chemical structure has been determined. Recently, insulin-like androgenic gland factor (IAG) was cloned from some decapod crustaceans. IAG shows sequence similarity to isopod AGHs, and has been suggested to be decapod AGH, although no direct evidence for the function of IAG has been reported. In order to elucidate the function of IAG, it is very important to analyze hemolymph levels of IAG during sex differentiation in decapod crustaceans. Therefore, in this study, we developed a sensitive and specific enzyme-linked immunosorbent assay (ELISA) for IAG of the Pacific grass shrimp Palaemon pacificus. A synthetic P. pacificus IAG B-chain was conjugated to keyhole limpet hemocyanin and injected into rabbits, and an antiserum against P. pacificus IAG was subsequently obtained. The synthetic P. pacificus IAG B-chain was labeled with horseradish peroxidase. Using the antiserum and labeled synthetic peptide, an ELISA system for P. pacificus IAG was developed. The sensitivity of the assay was 80 fmol/mL. The AG extract from P. pacificus produced a displacement curve which was almost parallel to that of the P. pacificus standard. The total amount of P. pacificus IAG in the AG was 32 fmol. This is the first report on the IAG content in the AG.
原著 2014 年度神奈川大学総合理学研究所共同研究助成論文

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