Departmental Bulletin Paper Citrobacter 属細菌による重金属還元特性

原, 大翼  ,  後反, 克典  ,  高山, 勝己

Sulfate-reducing bacteria (SRB), such as Desulfovibrio , produce hydrogen sulfi de by reducing sulfate in an anaerobic environment. Citrobacter murliniae SbR-43 , isolated from the surface soil in Fukui Prefecture as an antimony-resistant bacteria, also has sulfate-reduction ability. In this study,a variety of heavy metals—Fe3+, Co2+, Cu2+, Zn2+, and Cd2+—containing cultivation medium wereprecipitated as sulfi de compounds with the growth of Citrobacter murliniae SbR-43 . The evolution of the amount of H2S gas produced by Citrobacter murliniae SbR-43 is very high, and heavy metals such as Fe3+, Zn2+, and Cd2+ precipitated more than did E. coli . Resting cells of Citrobacter murliniae SbR-43 were coated with gold when incubated in an AuCl4- aqueous solution, and the morphologicalobservation of bacteria cells by SEM was easily possible without tedious conventional pretreatment,such as osmium staining.

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