Journal Article The impact of the host CCR5, TLR2 and TLR11 on production of nitric oxide, IL-6, IL-12 and growth of Toxoplasma gondii

Ibrahim, H. M.  ,  Nishikawa, Y

26 ( 1-2 )  , pp.18 - 29 , 2016-12 , National Research Center for Protozoan Diseases
ISSN:09174427
NCID:AA10824856
Description
The current study investigated the impact of the CCR5, TLR2 and TLR11 on production of nitric oxide (NO), IL-6 and IL-12 and growth of T. gondii, high virulent RH strain and avirulent PLK strain. All examined knockout macrophages infected with PLK strain produced significant lower levels of IL-12. On the other hand, TLR2 and TLR11 macrophages infected with RH strain produced significantly reduced levels of IL-12 as compared to wild-type macrophages. TLR2 and TLR11 macrophages infected with PLK strain significantly inhibited the production of IL-6 when compared to wild-type macrophages. Interestingly, significant reduction in the IL-6 and NO production was observed in TLR2 macrophages infected with PLK strain as compared to wild-type macrophages or to the other examined knockout macrophages. On contrary, significant increase in the NO levels was demonstrated in TLR11 macrophages infected with the RH strain. The growth of RH strain was significantly enhanced in CCR5, TLR2 and TLR11 macrophages as compared to wild-type macrophages. The highest parasite growth of both RH and PLK strains was achieved in TLR2 macrophages. In conclusion, the current study suggests that TLR2 is the most critical receptor in the host defense against T. gondii infection.
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