Departmental Bulletin Paper SDS-PAGEにおけるタンパク質の定量に関する適切な標準曲線関数の決定
The determination of the standard curve function for the protein quantity in SDS-PAGE

小澤 洋子  ,  君島 健夫  ,  小澤 秀夫

42pp.57 - 59 , 2018-03-20 , 神奈川工科大学
By the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the molecular weight and quantity of protein are determined. After the electrophoresis, gel is stained by coomassie brilliant blue (CBB) or other chemicals. The quantity of protein is determined by densitometry. In the low quantity below a few hundred nano grams of protein, the signal intensity is proportional to the quantity. By the increment of the quantity, the signal increment is suppressed. In the present report, the adequate standard curve function was determined, where the protein contents ranged from 0.020 to 10 μg.

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