Journal Article Live imaging of H3K9 acetylation in plant cells

Kurita, K.  ,  Kurita, K.  ,  Sakamoto, T.  ,  Sakamoto, T.  ,  Yagi, N.  ,  Yagi, N.  ,  Sakamoto, Y.  ,  Sakamoto, Y.  ,  Ito, A.  ,  Ito, A.  ,  Nishino, N.  ,  Nishino, N.  ,  Sako, K.  ,  Sako, K.  ,  Yoshida, M.  ,  Yoshida, M.  ,  木村, 宏  ,  Kimura, Hiroshi  ,  Seki, M.  ,  Seki, M.  ,  Matsunaga, S.  ,  Matsunaga, S.

7p.45894 , 2017-04
Proper regulation of histone acetylation is important in development and cellular responses to environmental stimuli. However, the dynamics of histone acetylation at the single-cell level remains poorly understood. Here we established a transgenic plant cell line to track histone H3 lysine 9 acetylation (H3K9ac) with a modification-specific intracellular antibody (mintbody). The H3K9ac-specific mintbody fused to the enhanced green fluorescent protein (H3K9ac-mintbody-GFP) was introduced into tobacco BY-2 cells. We successfully demonstrated that H3K9ac-mintbody-GFP interacted with H3K9ac in vivo. The ratio of nuclear/cytoplasmic H3K9ac-mintbody-GFP detected in quantitative analysis reflected the endogenous H3K9ac levels. Under chemically induced hyperacetylation conditions with histone deacetylase inhibitors including trichostatin A, Ky-2 and Ky-14, significant enhancement of H3K9ac was detected by H3K9ac-mintbody-GFP dependent on the strength of inhibitors. Conversely, treatment with a histone acetyltransferase inhibitor, C646 caused a reduction in the nuclear to cytoplasmic ratio of H3K9ac-mintbody-GFP. Using this system, we assessed the environmental responses of H3K9ac and found that cold and salt stresses enhanced H3K9ac in tobacco BY-2 cells. In addition, a combination of H3K9ac-mintbody-GFP with 5-ethynyl-2'-deoxyuridine labelling confirmed that H3K9ac level is constant during interphase.

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