Journal Article Corrosion Test Using Bottom Water from Oil-storage Tank and Microbial Community Analysis by Next Generation Sequencer

若井, 暁  ,  Wakai, Satoshi  ,  宮永, 一彦  ,  Miyanaga, Kazuhiko  ,  丹治, 保典  ,  TANJI, YASUNORI

64pp.540 - 544 , 2016-06 , Zairyo-to-Kankyo
The involvement of microorganisms in corrosion refers to microbiologically influenced corrosion (MIC). We have previously succeeded in a lab-scale corrosion test using bottom water from oil-storage tanks and shown the change in the microbial community after corrosion using denaturing gradient gel electrophoresis analysis. However, those community structures have not yet been correlated with accelerated corrosion. Therefore, we conducted further analysis of the microbial community by pyrosequencing using a next-generation sequencer. In the corrosion sample, an acetic acid bacterium Acetobacterium sp. and a sulfate-reducing bacterium Desulfovibrio sp. were enriched. In contrast, only Desulfovibrio sp. bacterium was enriched in the noncorroded culture, and Acetobacterium sp. bacterium was not observed. These results indicate that the Acetobacterium sp. bacterium may be involved in the accelerated corrosion. This is first report of corrosion by Acetobacterium sp. The combination of a lab-scale corrosion test and microbial community analysis by pyrosequencing would be useful to correlate between corrosion behavior and a change in microbial community. In the future, such investigation would contribute to understanding of MIC.

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