Departmental Bulletin Paper 〈原著論文〉細胞融合用基板としての細胞マイクロアレイへのPEG固定化と表面性状の評価

今城, 明美  ,  永岡, 志穂  ,  崔, 源換  ,  永島, 正嗣  ,  白石, 浩平

To prepare a surface-modified microarray(µAy) on both PEG segments and specific protein binding sites as a substrate for high-efficiency cell fusion, PEG macromonomer(macPEG) [P(macPEG)] or/and N-succinimidyl arylate(NAS) and macPEG copolymer [P(macPEG-co-NAS)] with different compositions were selectively immobilized on the glass spots of the µAy by using surface-initiated radical copolymerization. Two kinds of selective immobilization methods of 3-aminopropyltrimethoxysilane were attempted on a glass substrate as a model of spots of µAy by using 1)vapor deposition and 2)immersion in the solution, and evaluated their surfaces from the scanning probe microscope(SPM) and water contact angle measurements. Poly(macPEG) and poly-(macPEG-co-NAS) chains as brushes were observed on the glass(g-glass). As a result, the SPM images and wettability based on the immobilized polymers on the g-glasses were changed by changing treated methods of alkoxysilane.

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