紀要論文 〈Review〉Peripheral nerve storage and allotransplantation using green tea polyphenol in rats—its possibility for clinical application—Review of our works.

Kakinoki, Ryosuke  ,  Ikeguchi, Ryosuke  ,  Matsumoto, Taiichi  ,  Nakayama, Ken  ,  Fujita, Satoshi  ,  Ohta, Soichi  ,  Noguchi, Takashi  ,  Kaizawa, Yukitoshi  ,  Oda, Hiroki  ,  Yurie, Hirofumi  ,  Duncan, FM Scott  ,  Hyon, Suong-Hyu  ,  Tomiyama, Takashi  ,  Hashimoto, Kazuhiko  ,  Tanaka, Hiroki  ,  Nishimura, Shunji  ,  Akagi, Masao

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<Abstract>Background. We examined whether peripheral nerve segments immersed in polyphenol solution could be preserved and allotransplanted into rats without using immunosuppressants. Methods. Sciatic nerve segments, 20 mm long, were harvested from male Lewis or DA rats and transplanted into 15-mm sciatic nerve deficits in Lewis rats immediately, or after polyphenol treatment (immersion in 1 mg/mL polyphenol solution for one week and in Dulbecco's modified Eagle's medium for three weeks more), without using immunosuppressants. In the FA group, DA rat nerve segments were transplanted into Lewis rats immediately. In the PA group, DA rat nerve segments were transplanted into Lewis rats after polyphenol treatment. In the FI group, Lewis rat nerve segments were transplanted immediately into Lewis rats. In the PI group, Lewis rat nerve segments were transplanted into Lewis rats after polyphenol treatment. Nerve regeneration was assessed electrophysiologically and histomorphologically. Nerve segments from male Lewis or DA rats were also transplanted into female Lewis rats as in the PI, PA, FI, and FA groups. Genomic DNA was extracted from each transplanted nerve segment and subjected to polymerase chain reaction amplification specific for the sex-determining region of Y-chromosome and β-actin genes. Immunostaining for S-100 and glial fibrillary acidic protein, and Y-chromosome-specific in situ hybridization were also performed. Results. Nerve regeneration in the PI and PA groups was similar to that in the FI group and was significantly greater than that in the FA group. The ratios of the Sry/β-actin polymerase chain reaction products for PA, PI, FI, and FA groups were 0.144, 0.294, 0.615, and 0, respectively. Some of the S-100-positive and glial fibrillary acidic protein-positive cells displayed Sry signals in the PI and FI groups, but not in the FA group. Conclusion. Some Schwann cells survived for one month in polyphenol-treated nerve segments that were transplanted allogeneically without immunosuppressants.
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