Preparation of UCST types of nonion polymer modified glass and thermal stimuli-exfoliation of HeLa cells on the substrate
今城, 明典 ,
伊藤, 大時 ,
山田, 康枝白石, 浩平
近畿大学次世代基盤技術研究所報告 = Kinki University Research Institute of Fundamental Technology for Next Generation
59 , 2015-06-01 , 近畿大学次世代基盤技術研究所
Poly (acrylamide-co-acrylonitrile)[poly (AAm-co-AN)] with upper critical solution temperature (UCST) was grafted onto a glass substrate by surface initiated atom transfer radical polymerization(SI-ATRP). From the scanning probe microscope(SPM) measurements, poly-(AAm-co-AN) brushes were observed on the glass substrate(g-glass). The contact angle(θ) value of g-glass to water at 26℃ increased from θ=21°(the untreated glass) to θ=69°. On the other hand, that of g-glass at 40℃ decreased from θ=69°to θ=65°. After being HeLa cells attached and proliferated on the g-glass at 30℃ under UCST of poly(AAm-co-AN) burshes, the HeLa cells attached were thermally exfoliated by increasing culture temperature over 37℃ for 30 min in phosphate buffered saline(pH7.4). The % of mechanical and thermal stimuli-exfoliation of attached HeLa cells on the g-glass was about 90% despite of about 10% on untreated glass.