学術雑誌論文 INTRA-GOLGI CONNEXIN26 BEHAVES IN A PRO-ONCOGENIC MANNER IN HEAD AND NECK CANCER CELLS

IIKAWA, Nobuko  ,  YAMAMOTO, Yohei  ,  KAWASAKI, Yohei  ,  YOSHIOKA, Toshiaki  ,  NISHIJIMA, Aki  ,  ENOMOTO, Katsuhiko  ,  ISHIKAWA, Kazuo  ,  OMORI, Yasufumi

42 ( 2 )  , pp.87 - 94 , 2015-11-30 , 秋田医学会
ISSN:03866106
NII書誌ID(NCID):AN00009294
内容記述
Downregulation of gap junctional intercellular communication is an important hallmark of malignanttumours. One of the downregulation mechanisms is translocation of gap junction (GJ) proteincalled connexin from cell membrane into cytoplasm, nucleus, or Golgi apparatus. Interestingly, as tumours progress and reinforce their malignant phenotype, the amount of aberrantly-localised connexin increases in different cancers including oesophageal squamous cell carcinoma,thus suggesting that such an aberrantly-localised connexin should be oncogenic. Todetermine the roles of aberrantly-localised connexin in head and neck squamous cell carcinoma(HNSCC), we introduced wild-type connexin26 (wtCx26) or the Golgi-retained mutant Cx26(mtCx26) gene into human SAS lingual HNSCC cell line, which had lost the expression of connexinduring carcinogenesis. The wtCx26 protein was trafficked to cell membrane and formedGJ, which successfully exerted cell-cell communication. On the other hand, mtCx26 protein wasretained in the Golgi apparatus on the way to cell membrane. While the forced expression ofwtCx26 suppressed various malignant phenotypes including cell proliferation, motility, and invasivenessin vitro, mtCx26 significantly reinforced these phenotypes compared with the mock controlclone, indicating that an excessive accumulation of connexin protein in Golgi apparatus shouldbe involved in cancer progression.
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http://air.lib.akita-u.ac.jp/dspace/bitstream/10295/2987/1/akitai42-2%2887%29.pdf

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