||A HUMORAL LIPID PREVENTING FROM MOUSE ANAPHYLACTIC DEATH
MASUDA, Yutaka ,
KOYOTA, SouichiSUGIYAMA, Toshihiro
41 , 2015-06-30 , 秋田医学会
Anaphylaxis gives mice severe stress killing them. They could produce adaptogenicsubstance for coping the stress. In the present study, the adaptogenic substance preventing frommouse anaphylactic death was examined. Six-week-old male ddY mice were initially immunizedwith 1 mg/kg of ovalbumin and second immunized with the same dosage 10 days after the initialimmunization. The mice further immunized with the same dosage at 5 days or 15 days after thesecond immunization. All of the former were killed by the anaphylactic response, but none of thelatter were. Although reactivity of fucosylated antibodies inducing anaphylactic response wasnot different in the former sera and the latter sera, a lipid preventing from anaphylactic death wasfound in fraction eluted with 300 mM NaCl of the latter sera. The present findings suggest thatthe lipid had an adaptogenic activity against severe stress induced by anaphylactic response, andwas produced in late phase of the second immune response.