Departmental Bulletin Paper 大腸菌レスポンスレギュレーターFimZの機能解析

小川, 綾乃

 Bacteria are able to adapt to environmental changes by change of gene expression patterns in genome. Two-component regulatory system is major bacterial signal transduction system consists of a membrane-associating sensor kinase (SK) and a cytoplasmic response regulator(RR), which regulates gene expressions on its genome. On the E. coli K-12 genome, a total of 30 SKs and 32 RRs have been predicted. Among 32 RRs, 29 RRs are predicted to be DNA-binding transcription factors in which only FimZ has been uncharacterized. Recent studies indicate that FimZ induces the filamentation of E. coli cell at stationary phase. Transcriptome showed that FimZ induced genes involved in fimbria and putrescine syntheses and SOS response in E. coli. Furthermore, conserved aspartate residue on FimZ required for induction of fimbrial genes but not cell filamentation. rssB gene required for cell filamentation but not induction of fimbrial genes. Mass spectrometry detected AtpAD, ATP synthase, associating with FimZ. Taken all results together, I discuss the physiological and molecular function of E. coli FimZ at stationary phase. Key Words: Two-component system, FimZ, transcription factor, Escherichia coli

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