Departmental Bulletin Paper 大腸菌二成分制御系情報伝達ネットワークの研究

吉田, 美祐

Bacterial two-component system (TCS) is composed of the sensor kinase (SK) and the response regulator (RR). After monitoring an environmental signal or condition, SK activates RR through phosphorylation, ultimately leading to the signal-dependent regulation of genome transcription. In Escherichia coli, a total of more than 30 SK-RR pairs and a few orphan RRs exist. Cross talk of the signal transduction takes place at three stages: signal recognition by SK (stage 1); RR phosphorylation by SK (stage 2); and target recognition by RR (stage 3). For the elucidation of the entire signal transduction network of E. coli, I performed the comprehensive analysis of the stage-3 cross talk between six NarL-family RRs and between fourteen OmpR-family RRs. In addition, I investigated the role of uncharacterized RR YgeK. Results of both of in vivo and in vitro studies suggested that the stage-3 cross talk takes place through the not ambiguous but specific promoter recognition by RRs. Furthermore, I show that YgeK plays the important role for the biofilm formation and the acetate metabolism. These findings provide the insight into the perspective of TCS signal transduction network and contribute for understand the mechanism of the bacterial adaptation and survival against to environment change. Key Words: Two-component regulation system, Escherichia coli, cross talk, transcription factor

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