紀要論文 細菌走化性に関わる膜透過系因子の役割
INVOLVEMENT OF MEMBRANE TRANSPORTER COMPONENTS IN BACTERIAL CHEMOTAXIS

山元, 季実子  ,  YAMAMOTO, Kimiko

(56) 2015-03-24 , 法政大学大学院理工学・工学研究科
ISSN:2187-9923
NII書誌ID(NCID):AA12677220
内容記述
Bacterial chemotaxis has been recognized as one of the key properties in pathogenicity and bioremediation. Here I studied on roles of membrane transporter components in chemotaxis of two species, the pathogenic bacterium Vibrio cholerae (classical biotype) strain O395N1 and the pollutant-degrading soil bacterium Burkholderia sp. NK8. First, I found that two V. cholerae proteins, Mlp2 and Mlp3, with similarities to the major amino acid chemoreceptors, mediate responses to L-serine and amino acids with small R groups, respectively. Mlp3 did not bind L-serine but co-expression of a putative serine-binding protein (named SatA) with Mlp3 increased responses to L-serine, suggesting that SatA is a primary receptor and Mlp3 is a transducer to mediate serine response. Second, I found that 3-chlorobenzoate (3CB)-degrading Burkholderia sp. NK8 is attracted to 3CB and its degradation products, 3- and 4-chlorocatechol (3CC, and 4CC), and β-ketoadipate. These tactic abilities were ablished by curing of the megaplasmid pNK8. Taxis to β-ketoadipate was complemented by the introduction of a putative outer membrane porin gene ompNK8 found in pNK8, suggesting that the porin facilitates chemotaxis to β-ketoadipate.
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http://repo.lib.hosei.ac.jp/bitstream/10114/11064/1/11R9701%e5%b1%b1%e5%85%83%e5%ad%a3%e5%ae%9f%e5%ad%90.pdf

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