562015-03-24 , 法政大学大学院理工学・工学研究科
Some heavy metals are used as essential trace elements for plant growth. However, plants can’t grow in both excess and deficient concentrations of heavy metals. In this study, I examined the effect of excess heavy metal conditions on induction of hyper-sensitive cell death. The Arabidopsis thaliana cell-culture (MM2d) was exposed to the excess condition of five heavy metals (Cu, Fe, Zn, Mn and Cd ) and cell death was induced by application of a flagellin peptide 22 (flg22) that acted as a pathogen-associated molecular pattern triggering the immune response in plants. After application of flg22, I observed the rate of cell death, production of reactive oxygen species (ROS), gene expression of a pathogenesis-related gene of WRKY22 and superoxide dismutase (SOD) genes and finally the change of the amount of anti-oxidant substances. As a result, although significant differences were not observed between the control and these excess heavy metal conditions, repression of SOD gene expression by flg22 application suggested the involvement in the effective induction of hyper-sensitive cell death.