紀要論文 重金属過剰条件で培養したシロイヌナズナ細胞の過敏感反応の変化
EFFECT OF EXCESS HEAVY METAL CONDITIONS ON INDUCTION OF HYPER-SENSITIVE RESPONSE OF ARABIDOPSIS CELL CULTURE INDUCED BY APPLICATION OF A FLAGELLIN PEPTIDE

吉野, 加奈子  ,  YOSHINO, Kanako

(56) 2015-03-24 , 法政大学大学院理工学・工学研究科
ISSN:2187-9923
NII書誌ID(NCID):AA12677220
内容記述
Some heavy metals are used as essential trace elements for plant growth. However, plants can’t grow in both excess and deficient concentrations of heavy metals. In this study, I examined the effect of excess heavy metal conditions on induction of hyper-sensitive cell death. The Arabidopsis thaliana cell-culture (MM2d) was exposed to the excess condition of five heavy metals (Cu, Fe, Zn, Mn and Cd ) and cell death was induced by application of a flagellin peptide 22 (flg22) that acted as a pathogen-associated molecular pattern triggering the immune response in plants. After application of flg22, I observed the rate of cell death, production of reactive oxygen species (ROS), gene expression of a pathogenesis-related gene of WRKY22 and superoxide dismutase (SOD) genes and finally the change of the amount of anti-oxidant substances. As a result, although significant differences were not observed between the control and these excess heavy metal conditions, repression of SOD gene expression by flg22 application suggested the involvement in the effective induction of hyper-sensitive cell death.
本文を読む

http://repo.lib.hosei.ac.jp/bitstream/10114/10777/3/13R7208.pdf

このアイテムのアクセス数:  回

その他の情報