学位論文 Distribution of hemokinin-1 in the rat trigeminal ganglion and trigeminal sensory nuclear complex

Igawa, Kaori  ,  Funahashi, Hideki  ,  Miyahara, Yu  ,  Naono-Nakayama, Rumi  ,  Matsuo, Hisae  ,  Yamashita, Yoshihiro  ,  Sakoda, Sumio  ,  Nishimori, Toshikazu  ,  Ishida, Yasushi

2017-07 , Elsevier
ISSN:00039969
内容記述
学位論文の一部を構成しているため、http://hdl.handle.net/10458/6028に本文を掲載。
Objective: A new mammalian tachykinin peptide encoded in a TAC4 gene was identified and designated as hemokinin-1 (HK-1). A representative of the tachykinin peptide family is substance P (SP), and the function of SP has been well characterized as a pain transmitter or modulator, while it is possible that HK-1 is involved in pruriceptive processing, but, as yet, the distribution of HK-1 peptide in the trigeminal sensory system is still unknown. Thus, the aim of the present study was to elucidate the distribution of HK-1, while comparing the expression of SP, in the trigeminal ganglion and trigeminal sensory nuclear complex. ### Design: The trigeminal ganglion and the brain stem of male SD rats were used in the immunohistochemical study. Since the amino acid sequence in the carboxyl-terminal regions of HK-1 and SP is common, polyclonal antibodies of HK-1 and SP derived from 6 amino acids consisting of amino-terminal regions of these peptides were produced in guinea pig and rabbit, respectively. The immunohistochemical staining of HK-1 and SP was conducted using frozen sections of the trigeminal ganglion and brain stem in rats.Results: Immunohistochemical studies revealed the expression of HK-1 in small- and medium-sized trigeminal ganglion neurons, in the paratrigeminal nucleus, and in lamina I of the trigeminal nucleus caudalis, while there was no immunoreactivity of HK-1 in the trigeminal nucleus principalis, trigeminal nucleus oralis, and trigeminal nucleus interpolaris. ### Conclusion: These findings indicate that HK-1 is a target molecule for treatment of itch in the orofaicial regions.

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