学術雑誌論文 Evaluation of real-time PCR assay for the detection of Ascaris suum contamination in meat and organ meats

Yen Thi Hoang, Nguyen  ,  Zhenzhen Wang  ,  Maruyama, Haruhiko  ,  Horii, Yoichiro  ,  Nonaka, Nariaki  ,  Yoshida, Ayako

2017-03-24 , Wiley
ISSN:17454565
内容記述
学位論文の一部を構成しているため、http://hdl.handle.net/10458/6021に本文を掲載。
Ascarid larva migrans syndrome (ascarid LMS) is caused by ascarid roundworms including Ascaris suum. In East Asia, ascarid LMS has been considered as a food-borne disease in adulthood who has the dietary habitat of consuming raw or lightly cooked meat and organ meats. To evaluate the potential risk of A. suum infection from these foods, Ascaris specific real-time PCR was developed. The assay could constantly detect A. suum DNA up to 10 fg. Its specificity was confirmed by non-amplification with Toxocara canis and Toxocara cati DNA. A. suum DNA could be amplified from not only a single larva but also mouse liver spiked with a larva. Moreover, the assay could detect A. suum DNA in experimentally infected mouse liver, and showed higher sensitivity than a conventional digestion method. This real-time PCR assay would be useful for detecting the A. suum larval contamination in meat and organ meats.

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