||The detection of Propionibacterium acnes signatures in granulomas of lupus miliaris disseminatus faciei
Nishimoto, Junko ,
Amano, MasahiroSetoyama, Mitsuru
, p.418–421 , 2015-04 , Japanese Dermatological Association
Lupus miliaris disseminatus faciei is a papular eruption of the face in adults, which occurs predominantly on the lower eyelids. Histologically, the granulomatous lesions are primarily situated around the hair follicles, particularly the superficial region/infundibula. Its etiology remains to be elucidated. Propionibacterium acnes (P. acnes) has been recently suspected as a cause of sarcoidosis. In light of the sarcoid-like reactions that are present in lupus miliaris disseminatus faciei, we hypothesized that P. acnes may also be implicated in the granulomas associated with the disease. We evaluated nine DNA samples from the granulomatous lesions of the skin of the patients with lupus miliaris disseminatus faciei. We used laser capture microdissection to extract DNA from these regions. Polymerase chain reaction was performed to amplify segments of the 16S ribosomal RNA of P. acnes; the P. acnes gene was clearly detectable in all nine DNA samples. The gene was also detected in the samples from normal-appearing skin; however, these bands were faint in all samples. The results of the present study suggest that P. acnes plays one of the pathogenetic roles in lupus miliaris disseminatus faciei.