||Genotoxicity of occupational exposure of anesthesiologists to sevoflurane : The relationship between DNA damage and the work environment
Genotoxicity of occupational exposure of anesthesiologists to sevoflurane : The relationship between DNA damage and the work environment
大瀧, 恵 ,
川前, 金幸 ,
石川, 恵生小田, 真也
山形大学紀要. 医学 : 山形医学 = Bulletin of the Yamagata University. Medical science : Yamagata medical journal
91 , 2016-08-15 , 山形大学
Background: Inhalation anesthetics are often used for general anesthesia. However, several studies have reported that occupational exposure to inhalation anesthetics induces DNA damage and that sevoflurane anesthesia causes reversible genotoxic effects in patients. Our aim was to investigate the genotoxic effects in anesthesiologists of occupational exposure to sevoflurane, and to reveal the relevance of DNA damage and the work environment of anesthesiologists.Methods: Alkaline comet assay of the peripheral blood lymphocytes of 53 anesthesiologists (exposed group) and 37 office workers (control group) was performed, following which 100 cells were classified into 5 classes: 0: no migration, to 4: very high migration, the distance of migration being dependent on the degree of DNA damage. The total comet score (TCS) was calculated as n1+2×n2+3×n3+4×n4. Anesthesiologists were asked about their work environment.Results and Conclusions: TCSs were no different between the exposed and control groups (7.16±7.88 vs. 7.63±6.73, p=0.82) (95%CI 3.91-10.41 vs. 4.97-10.29). There was no significant association between TCS and the frequency of usage of sevoflurane. In conclusion, our study did not reveal DNA damage with chronic occupational exposure to sevoflurane.