Journal Article The CD45+ fraction in murine adipose tissue derived stromal cells harbors immune-inhibitory inflammatory cells

Nasti, Alessandro  ,  Sakai, Yoshio  ,  Seki, Akihiro  ,  Buffa, Geraldine Belen  ,  Komura, Takuya  ,  Mochida, Hatsune  ,  Yamato, Masatoshi  ,  Yoshida, Keiko  ,  Ho, Tuyen T. B.  ,  Takamura, Masayuki  ,  Usui, Soichiro  ,  Wada, Takashi  ,  Honda, Masao  ,  Kaneko, Shuichi

47 ( 12 )  , pp.2163 - 2174 , 2017-12 , Wiley
Stromal cells in adipose tissue are useful for repair/regenerative therapy as they harbor a substantial number of mesenchymal stem cells; therefore, freshly isolated autologous uncultured adipose tissue derived stromal cells (u-ADSCs) are useful for regenerative therapy, and obviate the need for mesenchymal stem cells. We evaluated the therapeutic effect of murine u-ADSCs and sorted subsets of u-ADSCs in a concanavalin A (ConA) induced murine model of hepatitis, as well as their characteristics. We found that 10–20% of u-ADSCs expressed the CD45 leukocyte-related antigen. CD68, which is a marker of macrophages (MΦs), was expressed by 50% of CD45+ u-ADSCs. About 90% of CD68+CD45+ cells expressed CD206 antigen, which is a marker of inhibitory M2-type MΦs. Genes related to M2-type MUs were especially more highly expressed by CD45+CD206+ u-ADSCs than by CD45− u-ADSCs. CD45+ u-ADSCs inhibited the expression of cytokines/chemokines and suppressed the proliferation of splenocytes stimulated with ConA. We observed that not only whole u-ADSCs, but also the CD45+ subset of u-ADSCs ameliorated the ConA-induced hepatitis in mice. In conclusion, we show that freshly isolated murine u-ADSCs were effective against acute hepatitis, and CD45+ u-ADSCs acting phenotypically and functionally like M2-type MΦs, contributed to the repair of liver tissue undergoing inflammation. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
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