||Renaturation of cytochrome C dissolved in polar phosphonate-type ionic liquids using highly polar zwitterions
Kuroda, Kosuke ,
Kohno, YukiOhno, Hiroyuki
872 , 2017-01-01 , Chemical Society of Japan = 日本化学会
We present a method for the renaturation of denatured cytochrome c in a phosphonate-type ionic liquid with the addition of a suitable zwitterion and a small amount of water. The polarity of zwitterions was found to be a critical property for the renaturation of cytochrome c. The renatured cytochrome c was reduced by the addition of sodium dithionite, suggesting that the renatured cytochrome c retains its redox ability. © 2017 The Chemical Society of Japan.