Journal Article Renaturation of cytochrome C dissolved in polar phosphonate-type ionic liquids using highly polar zwitterions

Kuroda, Kosuke  ,  Kohno, Yuki  ,  Ohno, Hiroyuki

46 ( 6 )  , pp.870 - 872 , 2017-01-01 , Chemical Society of Japan = 日本化学会
We present a method for the renaturation of denatured cytochrome c in a phosphonate-type ionic liquid with the addition of a suitable zwitterion and a small amount of water. The polarity of zwitterions was found to be a critical property for the renaturation of cytochrome c. The renatured cytochrome c was reduced by the addition of sodium dithionite, suggesting that the renatured cytochrome c retains its redox ability. © 2017 The Chemical Society of Japan.

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