||Time Course Effect of R-Alpha-Lipoic Acid on Cellular Metabolomics in Cultured Hepatoma Cells
Ikuta, Naoko ,
Chikamoto, Keita ,
Asano, Yuya ,
Yasui, Yoshiaki ,
Yokokawa, Haruka ,
Terao, Keiji ,
Rimbach, GeraldMatsugo, Seiichi
Journal of Medicinal Food
222 , 2017-03-01 , Mary Ann Liebert Inc.
Alpha-lipoic acid (LA) is a powerful antioxidant. LA has two enantiomers, R(+)-LA (R-LA) and S(-)-LA (S-LA). Of these, R-LA is naturally occurring and an essential cofactor in energy metabolism. R-LA treatment has been reported to affect glucose metabolism in rat hepatoma cells. This study analyzed the time course of metabolite levels in LA-treated cultured H4IIEC3 rat hepatoma cells, including a specific evaluation of the effect of R-LA and the enantioselectivity of LA. Principal component analysis showed that this experiment was well designed to observe enantioselectivity. R-LA treatment was found to inhibit the glycolysis and Thr-Gly-Ser pathways, as well as lactic acid production, leading to the inhibition of gluconeogenesis in starved H4IIEC3 cells. This study may provide mechanistic insight into how R-LA induces apoptosis in hepatoma cells. © Copyright 2017, Mary Ann Liebert, Inc. and Korean Society of Food Science and Nutrition 2017.
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