学術雑誌論文 Calcitonin-typical suppression of osteoclastic activity by amphioxus calcitonin superfamily peptides and insights into the evolutionary conservation and diversity of their structures

Sekiguchi, Toshio  ,  Shiraishi, Akira  ,  Satake, Honoo  ,  Kuwasako, Kenji  ,  Takahashi, Hiroki  ,  Sato, Masayuki  ,  Urata, Makoto  ,  Wada, Shuichi  ,  Endo, Masato  ,  Ikari, Takahiro  ,  Hattori, Atsuhiko  ,  Srivastav, Ajai K.  ,  Suzuki, Nobuo

246pp.294 - 300 , 2017-05-15 , Academic Press Inc. / Elsevier
ISSN:0016-6480
NII書誌ID(NCID):AA00654410
内容記述
Calcitonin (CT) is a hormone that decreases serum calcium level by suppressing osteoclastic activity in the vertebrate bone. In vertebrates, the structure-function relationship of CTs has been studied extensively. We recently identified three CT superfamily peptides, Bf-CTFP1 to 3, and clarified the molecular and functional characteristics of their receptor and receptor activity-modifying protein in amphioxus, Branchiostoma floridae. However, the CT activity of Bf-CTFPs has yet to be investigated. In the present study, a functional analysis of Bf-CTFPs was performed using goldfish scales having both osteoclasts and osteoblasts. All Bf-CTFPs suppressed osteoclastic activity via a goldfish CT receptor. Although the primary amino acid sequences of the Bf-CTFPs showed low sequence similarity to vertebrate CTs, Bf-CTFP1 to 3 share three amino acids, Thr25, Thr27, and Pro32-NH2, that are required for receptor binding, with salmon CT. Moreover, homology model analysis revealed that the Bf-CTFPs form alpha-helical structures. The alpha-helical position and length of Bf-CTFP1 and 2 were conserved with those of a highly potent ligand, teleost CT. Interestingly, the composition of the alpha-helix of Bf-CTFP3 differed from those of teleost CT, despite that the action of Bf-CTFP3 on goldfish scales was the same as that of Bf-CTFP1 and 2. Collectively, the present study provides new insights into the structure-function relationship of CT and its functional evolution in chordates. © 2017 Elsevier Inc.
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http://dspace.lib.kanazawa-u.ac.jp/dspace/bitstream/2297/46763/1/OC-PR-SUZUKI-N-294.pdf

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