Journal Article Tumor necrosis factor-α modifies the effects of Shiga toxin on glial cells

Leu, Hue  ,  Sugimoto, Naotoshi  ,  Shimizu, Masaki  ,  Toma, Tomoko  ,  Wada, Taizo  ,  Ohta, Kunio  ,  Yachie, Akihiro

38pp.139 - 143 , 2016-09-01 , Elsevier
Shiga toxin (STX) is one of the main factors inducing hemorrhagic colitis and hemolytic-uremic syndrome (HUS) in infections with STX-producing Escherichia coli (STEC). Approximately 62% of patients with HUS showed symptoms of encephalopathy in the 2011 Japanese outbreak of STEC infections. At that time, we reported elevated serum concentrations of tumor necrosis factor (TNF)-α in patients with acute encephalopathy during the HUS phase. In the current study, we investigated whether TNF-α augments the effects of STX in glial cell lines and primary glial cells. We found that TNF-α alone or STX in combination with TNF-α activates nuclear factor-κB (NF-κB) signaling and inhibits growth of glial cells. The magnitude of the NF-κB activation and the inhibition of cell growth by the STX and TNF-α combination was greater than that obtained with TNF-α alone or STX alone. Thus, this in vitro study reveals the role of TNF-α in glial cells during STEC infections. © 2016 Elsevier B.V. All rights reserved.

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