Departmental Bulletin Paper 肝細胞増殖因子の発現は骨格筋再生領域の拡大と同期する
Expression of hepatocyte growth factor is synchronized with expansion of myogenesis in regenerating skeletal muscle

田中, 正二  ,  河原, 榮  ,  中川, 敬夫

39 ( 2 )  , pp.85 - 94 , 2016-01-27 , 金沢大学つるま保健学会 = Tsuruma Health Science Society, Kanazawa University
The role of hepatocyte growth factor (HGF)-induced activation (phosphorylation) of its receptor, c-Met, and subsequent downstream signaling during muscle regeneration has not yet been adequately determined. We examined HGF and phospho-c-Met expression and localization during the expansion phase and time course of satellite cell activation, which is critical for muscle regeneration after injury. HGF, c-Met, myogenic differentiation 1 (MyoD), and myogenin mRNA levels were significantly increased during muscle regeneration after injury. Phospho-c-Met was localized on the surface of regenerating muscle cells. HGF was colocalized in the same cells as phospho-c-Met, MyoD, or myogenin. The muscle area occupied by HGF-, phospho-c-Met-, MyoD-, or myogenin-positive cells expanded along with expansion of regenerating muscle. Thus, c-Met is expressed in regenerating muscle cells immediately after injury, and its kinase activity is likely activated by HGF expressed by the same cells, suggesting that HGF and c-Met contribute to myogenesis in vivo.

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