学術雑誌論文 A novel in-frame deletion in the leucine zipper domain of C/EBPε leads to neutrophil-specific granule deficiency

Wada, Taizo  ,  Akagi, Tadayuki  ,  Muraoka, Masahiro  ,  Toma, Tomoko  ,  Kaji, Kenzo  ,  Agematsu, Kazunaga  ,  Koeffler, Phillip  ,  Yokota, Takashi  ,  Yachie, Akihiro

195 ( 1 )  , pp.80 - 86 , 2015-07-01 , American Association of Immunologists
ISSN:0022-1767
NII書誌ID(NCID):AA00699656
内容記述
Neutrophil-specific granule deficiency (SGD) is a rare autosomal recessive primary immunodeficiency characterized by neutrophil dysfunction, bilobed neutrophil nuclei and lack of neutrophil-specific granules. Defects in a myeloid-specific transcription factor, CCAAT/enhancer binding protein-ε (C/EBPε), have been identified in two cases in which homozygous frameshift mutations led to loss of the leucine zipper domain. In this study, we report a 55-y-old woman affected with SGD caused by a novel homozygous 2-aa deletion (ΔRS) in the leucine zipper domain of the C/EBPε gene. The patient showed characteristic neutrophil abnormalities and recurrent skin infections; however, there was no history of deep organ infections. Biochemical analysis revealed that, in contrast to the two frameshift mutations, the ΔRS mutant maintained normal cellular localization, DNA-binding activity, and dimerization, and all three mutants exhibited marked reduction in transcriptional activity. The ΔRS mutant was defective in its association with Gata1 and PU.1, as well as aberrant cooperative transcriptional activation of eosinophil major basic protein. Thus, the ΔRS likely impairs protein-protein interaction with other transcription factors, resulting in a loss of transcriptional activation. These results further support the importance of the leucine zipper domain of C/EBPε for its essential function, and indicate that multiple molecular mechanisms lead to SGD. © 2015 by The American Association of Immunologists, Inc.
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http://dspace.lib.kanazawa-u.ac.jp/dspace/bitstream/2297/43044/1/HO-PR-WADA-T-80.pdf

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