Journal Article Effect of sub- and supercritical water treatments on the physicochemical properties of crab shell chitin and its enzymatic degradation

Osada, Mitsumasa  ,  MIURA, Chika  ,  NAKAGAWA, Yuko S.  ,  KAIHARA, Mikio  ,  NIKAIDO, Mitsuru  ,  TOTANI, Kazuhide

134pp.718 - 725 , 2015-12-10 , ELSEVIER
NCID:AA10626772
Description
This study examined the effects of sub- and supercritical water pretreatments on the physicochemical properties of crab shell α-chitin and its enzymatic degradation to obtain N,N′-diacetylchitobiose (GlcNAc)2. Following sub- and supercritical water pretreatments, the protein in the crab shell was removed and the residue of crab shell contained α-chitin and CaCO3. Prolonged pretreatment led to α-chitin decomposition. The reaction of pure α-chitin in sub- and supercritical water pretreatments was investigated separately; we observed lower mean molecular weight and weaker hydrogen bonds compared with untreated α-chitin. (GlcNAc)2 yields from enzymatic degradation of subcritical (350 °C, 7 min) and supercritical water (400 °C, 2.5 min) pretreated crab shell were 8% and 6%, compared with 0% without any pretreatment. This study shows that sub- and supercritical water pretreatments of crab shell provide to an alternative method to the use of acid and base for decalcification and deproteinization of crab shell required for (GlcNAc)2 production.
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