Journal Article Fast epi-detected broadband multiplex CARS and SHG imaging of mouse skull cells

Capitaine, Erwan  ,  Moussa, Nawel Ould  ,  Louot, Christophe  ,  Bardet, Sylvia M.  ,  Kano, Hideaki  ,  Duponchel, Ludovic  ,  Lévêque, Philippe  ,  Couderc, Vincent  ,  Leproux, Philippe

9 ( 1 )  , pp.245 - 253 , 2017-12 , Optical Society of America
We present a bimodal imaging system able to obtain epi-detected mutiplex coherent anti-Stokes Raman scattering (M-CARS) and second harmonic generation (SHG) signals coming from biological samples. We studied a fragment of mouse parietal bone and could detect broadband anti-Stokes and SHG responses originating from bone cells and collagen respectively. In addition we compared two post-processing methods to retrieve the imaginary part of the third-order nonlinear susceptibility related to the spontaneous Raman scattering.

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