Journal Article Exploration of Spinal Cord Aging–Related Proteins Using a Proteomics Approach

Koda, Masao  ,  Kamiya, Koshiro  ,  Furuya, Takeo  ,  Hashimoto, Masayuki  ,  Mannoji, Chikato  ,  Inada, Taigo  ,  Ota, Mitsutoshi  ,  Maki:, Satoshi  ,  Ijima, Yasushi  ,  Saito, Junya  ,  Kitamura, Mitsuhiro  ,  Ohtori, Seiji  ,  Orita, Sumihisa  ,  Inage, Kazuhide  ,  Yamazaki, Masashi

11p.UNSP 1179069517713019 , 2017-06 , SAGE Publishing
ISSN:1179-0695
Description
How aging affects the spinal cord at a molecular level is unclear. The aim of this study was to explore spinal cord aging–related proteins that may be involved in pathological mechanisms of age-related changes in the spinal cord. Spinal cords of 2-year-old and 8-week-old female Sprague-Dawley rats were dissected from the animals. Protein samples were subjected to 2-dimentional polyacrylamide gel electrophoresis followed by mass spectrometry. Screened proteins were further investigated with immunohistochemistry and Western blotting. Among the screened proteins, we selected α-crystallin B-subunit (αB-crystallin) and peripherin for further investigation because these proteins were previously reported to be related to central nervous system pathologies. Immunohistochemistry and Western blotting revealed significant upregulation of αB-crystallin and peripherin expression in aged rat spinal cord. Further exploration is needed to elucidate the precise mechanism and potential role of these upregulated proteins in spinal cord aging processes.
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