Journal Article Functional Analysis of Dendritic Cells Generated from T-iPSCs from CD4+ T Cell Clones of Sjögren's Syndrome

Iizuka-Koga, Mana  ,  Asashima, Hiromitsu  ,  Ando, Miki  ,  Lai, Chen-Yi  ,  Mochizuki, Shinji  ,  Nakanishi, Mahito  ,  Nishimura, Toshinobu  ,  Tsuboi, Hiroto  ,  Hirota, Tomoya  ,  Takahashi, Hiroyuki  ,  Matsumoto, Isao  ,  Otsu, Makoto  ,  Sumida, Takayuki

8 ( 5 )  , pp.1155 - 1163 , 2017-05 , Cell Press , Elsevier Inc.
Although it is important to clarify the pathogenic functions of T cells in human samples, their examination is often limited due to difficulty in obtaining sufficient numbers of dendritic cells (DCs), used as antigen-presenting cells, especially in autoimmune diseases. We describe the generation of DCs from induced pluripotent stem cells derived from T cells (T-iPSCs). We reprogrammed CD4+ T cell clones from a patient with Sjögren's syndrome (SS) into iPSCs, which were differentiated into DCs (T-iPS-DCs). T-iPS-DCs had dendritic cell-like morphology, and expressed CD11c, HLA-DR, CD80, CD86, and also BDCA-3. Compared with monocyte-derived DCs, the capacity for antigen processing was similar, and T-iPS-DCs induced the proliferative response of autoreactive CD4+ T cells. Moreover, we could evaluate T cell functions of the patient with SS. In conclusion, we obtained adequate numbers of DCs from T-iPSCs, which could be used to characterize pathogenic T cells in autoimmune diseases such as SS.

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