学術雑誌論文 Electrochemical Control of Bioluminescence by Blocking the Adsorption of the Bacterial Luciferase Using a Mercaptobipyridine Self-assembled Monolayer

YAMASAKI, Shinya  ,  YAMADA, Shuto  ,  TAKEMURA, Hiroyuki  ,  TAKEHARA, Kô

33 ( 3 )  , pp.307 - 311 , 2017-03 , The Japan Society for Analytical Chemistry
ISSN:0910-6340
NII書誌ID(NCID):AA10500785
内容記述
An N-butyl-N′-(4-mercaptobutyl)-4,4′-bipyridinium (4BMBP) was modified on a gold electrode to improve the electrochemical control of the bacterial luciferase (BL) luminescence system. The 4BMBP-modified gold electrode (4BMBP/Au) was able to prevent the adsorption of BL on the electrode surface, and enhanced the electrochemical regeneration rate of the reduced flavin mononucleotide (FMNH2), which is one of the substrates of the BL luminescence reaction. By using the 4BMBP/Au, the luminescence intensity increased by about 27% compared to that of a bare gold electrode (bare Au). Moreover, the modified electrode improved the time required for analysis because the modified layer prevented BL adsorption. Even without a refreshing procedure for each measurement, a constant luminescence intensity could be observed, and the analysis time was reduced to half (about 10 min) for one sample. The 4BMBP/Au is not only useful to control of the BL luminescence system, but also for electrochemical measurements in the presence of proteins.
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