学術雑誌論文 Discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde

Doi, Shiori  ,  Hashimoto, Yoshiteru  ,  Tomita, Chiaki  ,  Kumano, Takuto  ,  Kobayashi, Michihiko

6p.38021 , 2016-12 , Nature Publishing Group
ISSN:2045-2322
内容記述
Piperonal-catabolizing microorganisms were isolated from soil, the one (strain CT39-3) exhibiting the highest activity being identified as Burkholderia sp. The piperonal-converting enzyme involved in the initial step of piperonal metabolism was purified from strain CT39-3. Gene cloning of the enzyme and a homology search revealed that the enzyme belongs to the xanthine oxidase family, which comprises molybdoenzymes containing a molybdopterin cytosine dinucleotide cofactor. We found that the piperonal-converting enzyme acts on piperonal in the presence of O2, leading to formation of piperonylic acid and H2O2. The growth of strain CT39-3 was inhibited by higher concentrations of piperonal in the culture medium. Together with this finding, the broad substrate specificity of this enzyme for various aldehydes suggests that it would play an important role in the defense mechanism against antimicrobial compounds derived from plant species.
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