学術雑誌論文 pp32 and APRIL are host cell-derived regulators of influenza virus RNA synthesis from cRNA

Sugiyama, Kenji  ,  Kawaguchi, Atsushi  ,  Okuwaki, Mitsuru  ,  Nagata, Kyosuke

4p.e08939 , 2015-10 , eLife Sciences Publications, Ltd
ISSN:2050-084x
内容記述
Replication of influenza viral genomic RNA (vRNA) is catalyzed by viral RNA-dependent RNA polymerase (vRdRP). Complementary RNA (cRNA) is first copied from vRNA, and progeny vRNAs are then amplified from the cRNA. Although vRdRP and viral RNA are minimal requirements, efficient cell-free replication could not be reproduced using only these viral factors. Using a biochemical complementation assay system, we found a novel activity in the nuclear extracts of uninfected cells, designated IREF-2, that allows robust unprimed vRNA synthesis from a cRNA template. IREF-2 was shown to consist of host-derived proteins, pp32 and APRIL. IREF-2 interacts with a free form of vRdRP and preferentially upregulates vRNA synthesis rather than cRNA synthesis. Knockdown experiments indicated that IREF-2 is involved in in vivo viral replication. On the basis of these results and those of previous studies, a plausible role(s) for IREF-2 during the initiation processes of vRNA replication is discussed.
本文を読む

https://tsukuba.repo.nii.ac.jp/?action=repository_action_common_download&item_id=38290&item_no=1&attribute_id=17&file_no=1

このアイテムのアクセス数:  回

その他の情報