Journal Article Root endophytes enhance stress-tolerance of Cicuta virosa L. growing in a mining pond of eastern Japan

Nagata, Satoshi  ,  Yamaji, Keiko  ,  Nomura, Nobuhiko  ,  Ishimoto, Hiroshi

30 ( 2 )  , pp.116 - 125 , 2015-04 , Wiley
Cicuta virosa L. plants can grow in a pond subjected to heavy-metal inputs at the Hitachi mine, eastern Japan. They accumulate heavy-metal elements, especially high concentrations of zinc (Zn), in their roots. We focused on the role that root bacterial endophytes play in the heavy-metal uptake of plants and the provision of heavy-metal tolerance within plants. Our purpose was to clarify the effects of endophytes on: (i) Zn accumulation in C. virosa roots; (ii) growth of C. virosa seedlings; and (iii) heavy-metal tolerance of C. virosa plants. Root endophytic Pseudomonas putida and Rhodopseudomonas sp., which induced the high production of Zn-chelating compounds, were selected for the seedling inoculation test. The results of the inoculation test demonstrated that both strains of endophytes increased Zn accumulation in C. virosa roots by solubilizing Zn in the sediment. Both strains also increased the growth of seedlings by possible production of indole-3-acetic acid in the plant. The heavy-metal tolerance of C. virosa seedlings was likely promoted by producing metal-chelating compounds that detoxify the metals in the plant tissues, and by decreasing the heavy-metal contents in the tissues via rapid seedling growth. Thus, such mutualistic interactions between plants and bacteria contribute to the persistence of C. virosa in this severe environment.

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