||Electron transfer pathways in a multiheme cytochrome MtrF
Watanabe, Hiroshi C. ,
Yamashita, YukiIshikita, Hiroshi
Proceedings of the National Academy of Sciences of the United State of America Online Edition
2921 , 2017-03-14 , National Academy of Sciences
In MtrF, an outer-membrane multiheme cytochrome, the 10 heme groups are arranged in heme binding domains II and IV along the pseudo-C2 axis, forming the electron transfer (ET) pathways. Previous reports based on molecular dynamics simulations showed that the redox potential (Em) values for the heme pairs located in symmetrical positions in domains II and IV were similar, forming bidirectional ET pathways [Breuer M, Zarzycki P, Blumberger J, Rosso KM (2012) J Am Chem Soc 134(24):9868–9871]. Here, we present the Em values of the 10 hemes in MtrF, solving the linear Poisson–Boltzmann equation and considering the protonation states of all titratable residues and heme propionic groups. In contrast to previous studies, the Em values indicated that the ET is more likely to be downhill from domain IV to II because of localization of acidic residues in domain IV. Reduction of hemes in MtrF lowered the Em values, resulting in switching to alternative downhill ET pathways that extended to the flavin binding sites. These findings present an explanation of how MtrF serves as an electron donor to extracellular substrates.
UTokyo Research掲載「天然のダイオードタンパク質」 URI: http://www.u-tokyo.ac.jp/ja/utokyo-research/research-news/Protein-acts-as-natural-diode.html
UTokyo Research "Protein acts as natural diode" URI: http://www.u-tokyo.ac.jp/en/utokyo-research/research-news/Protein-acts-as-natural-diode.html