Journal Article Energetics of proton release on the first oxidation step in the water-oxidizing enzyme

Saito, Keisuke  ,  Rutherford, A. William  ,  Ishikita, Hiroshi

6p.8488 , 2015-10-07 , Nature Publishing Group
ISSN:2041-1723 (online)
In photosystem II (PSII), the Mn4CaO5 cluster catalyses the water splitting reaction. The crystal structure of PSII shows the presence of a hydrogen-bonded water molecule directly linked to O4. Here we show the detailed properties of the H-bonds associated with the Mn4CaO5 cluster using a quantum mechanical/molecular mechanical approach. When O4 is taken as a μ-hydroxo bridge acting as a hydrogen-bond donor to water539 (W539), the S0 redox state best describes the unusually short O4–OW539 distance (2.5 Å) seen in the crystal structure. We find that in S1, O4 easily releases the proton into a chain of eight strongly hydrogen-bonded water molecules. The corresponding hydrogen-bond network is absent for O5 in S1. The present study suggests that the O4-water chain could facilitate the initial deprotonation event in PSII. This unexpected insight is likely to be of real relevance to mechanistic models for water oxidation.
UTokyo Research掲載「光合成の水分解反応初期に水素イオンが放出される仕組みを解明」 URI:
UTokyo Research "Pathway for initial proton released from water-oxidizing enzyme" URI:

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