紀要論文 観察教材としてのミズカビの培養法の改良
Modification of Culturing Method for Saprolegnia sp. as the Observation Material in Biology

竹下, 俊治  ,  高尾, 彰

23pp.27 - 30 , 2017-03-22 , 広島大学大学院教育学研究科附属教育実践総合センター
ISSN:1341-111X
NII書誌ID(NCID):AN10491493
内容記述
Saprolegnia spp., common aquatic fungi, are known as one of the appropriate material to observe the reproductive process in its life cycle. The aim of the present study is to modify culturing method of Saprolegnia sp. We prepared culture media containing various concentration of the Cannabis extract solidified by agar. The hyphae of Saprolegnia sp. were cultured in sterilized water with small pieces of the agar media. As the result, many zoosporangia and archegonia were observed under the light microscope. In comparison with the ordinary culturing method using whole cotyledon of Cannabis, the colonies of Saprolegnia sp. obtained by this modified method were suitable to observe various stage of life cycle. The nutritional conditions in culturing are able to be controlled by this method. Therefore, it will be used as the practical method in the biology class.
本研究は,科学研究費基盤研究(A)(一般)(25242015)および科学研究費基盤研究(C)(一般)(26350235)の助成を受けて行った。
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http://ir.lib.hiroshima-u.ac.jp/files/public/4/42769/20170414110438427887/HiroshimaJSchEduc_23_27.pdf

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