Departmental Bulletin Paper 簡易蛍光顕微鏡の開発と高等学校生物における活用の提案
Development of a simple, hand made fluorescence microscope and a proposal to its practical use for the high school biology

竹下, 俊治  ,  雑賀, 大輔  ,  間賀, 綾音  ,  絹谷, 和子  ,  福田(時澤), 味佳

22pp.143 - 148 , 2016-03-22 , 広島大学大学院教育学研究科附属教育実践総合センター
In the present study, a simple hand made fluorescence microscope was developed. Four UV-LED lamps (NS360L-5RLO, Nitride Semiconductors Co., Ltd.) were used for the ultraviolet irradiation device. Some problems of a sample device have been improved and that has been completed. As the result, the nuclear fluorescence of the epidermal tissue of Tradescantia ohiensis stained by DAPI (4',6-diamidino-2-phenylindole) were observed. This low cost simple fluorescence microscope was very effective for the student’s activities in the high school biology classes. In this paper, authors proposed an example of experiment which analyze relative DNA content value of nucleus in mitotic cell cycle by using this fluorescence microscope.

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