Journal Article Low Cell-Matrix Adhesion Reveals Two Subtypes of Human Pluripotent Stem Cells

Yu, Leqian  ,  Li, Junjun  ,  Hong, Jiayin  ,  Takashima, Yasuhiro  ,  Fujimoto, Nanae  ,  Nakajima, Minako  ,  Yamamoto, Akihisa  ,  Dong, Xiaofeng  ,  Dang, Yujiao  ,  Hou, Yu  ,  Yang, Wei  ,  Minami, Itsunari  ,  Okita, Keisuke  ,  Tanaka, Motomu  ,  Luo, Chunxiong  ,  Tang, Fuchou  ,  Chen, Yong  ,  Tang, Chao  ,  Kotera, Hidetoshi  ,  Liu, Li

11 ( 1 )  , pp.142 - 156 , 2018-07-10 , Elsevier BV
ゼラチンナノファイバー培養基材を用いて ヒトiPS細胞から多能性能が高い細胞種の単離に成功 --高品質なiPS細胞の供給に期待--. 京都大学プレスリリース. 2018-07-04.
We show that a human pluripotent stem cell (hPSC) population cultured on a low-adhesion substrate developed two hPSC subtypes with different colony morphologies: flat and domed. Notably, the dome-like cells showed higher active proliferation capacity and increased several pluripotent genes’ expression compared with the flat monolayer cells. We further demonstrated that cell-matrix adhesion mediates the interaction between cell morphology and expression of KLF4 and KLF5 through a serum response factor (SRF)-based regulatory double loop. Our results provide a mechanistic view on the coupling among adhesion, stem cell morphology, and pluripotency, shedding light on the critical role of cell-matrix adhesion in the induction and maintenance of hPSC.

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