学術雑誌論文 Establishment of Immunodeficient Retinal Degeneration Model Mice and Functional Maturation of Human ESC-Derived Retinal Sheets after Transplantation

Iraha, Satoshi  ,  Tu, Hung-Ya  ,  Yamasaki, Suguru  ,  Kagawa, Takahiro  ,  Goto, Motohito  ,  Takahashi, Riichi  ,  Watanabe, Takehito  ,  Sugita, Sunao  ,  Yonemura, Shigenobu  ,  Sunagawa, Genshiro A.  ,  Matsuyama, Take  ,  Fujii, Momo  ,  Kuwahara, Atsushi  ,  Kishino, Akiyoshi  ,  Koide, Naoshi  ,  Eiraku, Mototsugu  ,  Tanihara, Hidenobu  ,  Takahashi, Masayo  ,  Mandai, Michiko

10pp.1059 - 1074 , 2018-03-13 , Elsevier BV
ISSN:2213-6711
内容記述
Increasing demand for clinical retinal degeneration therapies featuring human ESC/iPSC-derived retinal tissue and cells warrants proof-of-concept studies. Here, we established two mouse models of end-stage retinal degeneration with immunodeficiency, NOG-rd1-2J and NOG-rd10, and characterized disease progress and immunodeficient status. We also transplanted human ESC-derived retinal sheets into NOG-rd1-2J and confirmed their long-term survival and maturation of the structured graft photoreceptor layer, without rejection or tumorigenesis. We recorded light responses from the host ganglion cells using a multi-electrode array system; this result was consistent with whole-mount immunostaining suggestive of host-graft synapse formation at the responding sites. This study demonstrates an application of our mouse models and provides a proof of concept for the clinical use of human ESC-derived retinal sheets.
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http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/230369/1/j.stemcr.2018.01.032.pdf

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