学術雑誌論文 Enhanced Therapeutic Effects of Human iPS Cell Derived-Cardiomyocyte by Combined Cell-Sheets with Omental Flap Technique in Porcine Ischemic Cardiomyopathy Model

Kawamura, Masashi  ,  Miyagawa, Shigeru  ,  Fukushima, Satsuki  ,  Saito, Atsuhiro  ,  Miki, Kenji  ,  Funakoshi, Shunsuke  ,  Yoshida, Yoshinori  ,  Yamanaka, Shinya  ,  Shimizu, Tatsuya  ,  Okano, Teruo  ,  Daimon, Takashi  ,  Toda, Koichi  ,  Sawa, Yoshiki

72017-08-18 , Springer Nature
ISSN:2045-2322
内容記述
Transplant of human induced pluripotent stem cell derived cardiomyocytes (hiPS-CMs) cell-sheet is a promising approach for treating ischemic cardiomyopathy (ICM). However, poor blood supply to the transplanted cell-sheet is a concern related to the effectiveness and durability of the treatment. Herein, we hypothesized that the combined the omentum flap might enhance survival and the therapeutic effects of hiPS-CM cell-sheet transplant for ICM treatment. Treatment by Wnt signaling molecules in hiPS cells produced hiPS-CMs, which were magnetically labeled by superparamagnetic iron oxide (SPIO), followed by culture in the thermoresponsive dishes to generate hiPS-CMs cell-sheets. A porcine ICM model included 4 groups; sham operation, omentum flap only, cell-sheet only, or combination therapy. Ejection fraction (EF) was significantly greater in the cell-sheet only and combination group compared to the other groups during the follow-up period. At 3 months, the EF of the combination group was significantly greater than that of the cell-sheet only group. Consistently, the survival rate of the SPIO-labeled hiPS-CMs, as assessed by MRI, was significantly greater in the combination group than in the cell-sheet only group. This cell delivery system would be useful in optimizing the hiPS-CM cell-sheet transplant for treating severe heart failure.
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http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/228151/1/s41598-017-08869-z.pdf

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