Journal Article Structural and spatial chromatin features at developmental gene loci in human pluripotent stem cells

Ikeda, Hiroki  ,  Sone, Masamitsu  ,  Yamanaka, Shinya  ,  Yamamoto, Takuya

82017-11-20 , Springer Nature
多能性幹細胞における分化関連遺伝子座の染色体高次構造と核内配置の同定. 京都大学プレスリリース. 2017-11-20.
Higher-order chromatin organization controls transcriptional programs that govern cell properties and functions. In order for pluripotent stem cells (PSCs) to appropriately respond to differentiation signals, developmental gene loci should be structurally and spatially regulated to be readily available for immediate transcription, even though these genes are hardly expressed in PSCs. Here, we show that both chromatin interaction profiles and nuclear positions at developmental gene loci differ between human somatic cells and hPSCs, and that changes in the chromatin interactions are closely related to the nuclear repositioning. Moreover, we also demonstrate that developmental gene loci, which have bivalent histone modifications, tend to colocalize in PSCs. Furthermore, this colocalization requires PRC1, PRC2, and TrxG complexes, which are essential regulatory factors for the maintenance of transcriptionally poised developmental genes. Our results indicate that higher-order chromatin regulation may be an integral part of the differentiation capacity that defines pluripotency.

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