学術雑誌論文 Replications of Two Closely Related Groups of Jumbo Phages Show Different Level of Dependence on Host-encoded RNA Polymerase

Matsui, Takeru  ,  Yoshikawa, Genki  ,  Mihara, Tomoko  ,  Chatchawankanphanich, Orawan  ,  Kawasaki, Takeru  ,  Nakano, Miyako  ,  Fujie, Makoto  ,  Ogata, Hiroyuki  ,  Yamada, Takashi

82017-06-13 , Frontiers Media SA
ISSN:1664-302X
内容記述
Ralstonia solanacearum phages ΦRP12 and ΦRP31 are jumbo phages isolated in Thailand. Here we show that they exhibit similar virion morphology, genome organization and host range. Genome comparisons as well as phylogenetic and proteomic tree analyses support that they belong to the group of ΦKZ-related phages, with their closest relatives being R. solanacearum phages ΦRSL2 and ΦRSF1. Compared with ΦRSL2 and ΦRSF1, ΦRP12 and ΦRP31 possess larger genomes (ca. 280 kbp, 25% larger). The replication of ΦRP12 and ΦRP31 was not affected by rifampicin treatment (20 μg/ml), suggesting that phage-encoded RNAPs function to start and complete the infection cycle of these phages without the need of host-encoded RNAPs. In contrast, ΦRSL2 and ΦRSF1, encoding the same set of RNAPs, did not produce progeny phages in the presence of rifampicin (5 μg/ml). This observation opens the possibility that some ΦRP12/ΦRP31 factors that are absent in ΦRSL2 and ΦRSF1 are involved in their host-independent transcription.
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http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/226642/1/fmicb.2017.01010.pdf

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