学術雑誌論文 Vinculin association with actin cytoskeleton is necessary for stiffness-dependent regulation of vinculin behavior

Omachi, Tomohiro  ,  Ichikawa, Takafumi  ,  Kimura, Yasuhisa  ,  Ueda, Kazumitsu  ,  Kioka, Noriyuki

12 ( 4 ) 2017-04-01 , Public Library of Science
ISSN:1932-6203
内容記述
The extracellular matrix (ECM) is a major regulator of cell behavior. Recent studies have indicated the importance of the physical properties of the ECM, including its stiffness, for cell migration and differentiation. Using actomyosin-generated forces, cells pull the ECM and sense stiffness via cell-ECM adhesion structures called focal adhesions (FAs). Vinculin, an actin-binding FA protein, has emerged as a major player in FA-mediated mechanotransduction. Although vinculin is important for sensing ECM stiffness, the role of vinculin binding to actin in the ECM stiffness-mediated regulation of vinculin behavior remains unknown. Here, we show that an actin binding-deficient mutation disrupts the ECM stiffness-dependent regulation of CSB (cytoskeleton stabilization buffer) resistance and the stable localization of vinculin. These results suggest that the vinculin-actin interaction participates in FA-mediated mechanotransduction.
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http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/225270/1/journal.pone.0175324.pdf

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