Journal Article New platform for simple and rapid protein-based affinity reactions

Kubota, Kei  ,  Kubo, Takuya  ,  Tanigawa, Tetsuya  ,  Naito, Toyohiro  ,  Otsuka, Koji

Scientific Reports
7 ( 1 ) 2017-03-14 , Springer Nature
ISSN:2045-2322
Description
タンパク質間反応のための新規プラットフォーム : 抗体分離の簡便化・高速化・低コスト化に向けて. 京都大学プレスリリース. 2017-03-15.
We developed a spongy-like porous polymer (spongy monolith) consisting of poly (ethylene-co-glycidyl methacrylate) with continuous macropores that allowed efficient in situ reaction between the epoxy groups and proteins of interest. Immobilization of protein A on the spongy monolith enabled high-yield collection of immunoglobulin G (IgG) from cell culture supernatant even at a high flow rate. In addition, immobilization of pepsin on the spongy monolith enabled efficient online digestion at a high flow rate.
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http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/218919/1/s41598-017-00264-y.pdf

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