学術雑誌論文 SOX2 O-GlcNAcylation alters its protein-protein interactions and genomic occupancy to modulate gene expression in pluripotent cells

Myers, Samuel A.  ,  Peddada, Sailaja  ,  Chatterjee, Nilanjana  ,  Friedrich, Tara  ,  Tomoda, Kiichrio  ,  Krings, Gregor  ,  Thomas, Sean  ,  Maynard, Jason  ,  Broeker, Michael  ,  Thomson, Matthew  ,  Pollard, Katherine  ,  Yamanaka, Shinya  ,  Burlingame, Alma L.  ,  Panning, Barbara

52016-03-07 , eLife Sciences Publications Ltd
ISSN:2050-084X
内容記述
The transcription factor SOX2 is central in establishing and maintaining pluripotency. The processes that modulate SOX2 activity to promote pluripotency are not well understood. Here, we show SOX2 is O-GlcNAc modified in its transactivation domain during reprogramming and in mouse embryonic stem cells (mESCs). Upon induction of differentiation SOX2 O-GlcNAcylation at serine 248 is decreased. Replacing wild type with an O-GlcNAc-deficient SOX2 (S248A) increases reprogramming efficiency. ESCs with O-GlcNAc-deficient SOX2 exhibit alterations in gene expression. This change correlates with altered protein-protein interactions and genomic occupancy of the O-GlcNAc-deficient SOX2 compared to wild type. In addition, SOX2 OGlcNAcylation impairs the SOX2-PARP1 interaction, which has been shown to regulate ESC selfrenewal. These findings show that SOX2 activity is modulated by O-GlcNAc, and provide a novel regulatory mechanism for this crucial pluripotency transcription factor.
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http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/218635/1/eLife.10647.pdf

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