学術雑誌論文 Subtle modulation of ongoing calcium dynamics in astrocytic microdomains by sensory inputs.

Asada, Akiko  ,  Ujita, Sakiko  ,  Nakayama, Ryota  ,  Oba, Shigeyuki  ,  Ishii, Shin  ,  Matsuki, Norio  ,  Ikegaya, Yuji

3 ( 10 ) 2015-10-04 , Wiley-Blackwell
ISSN:2051-817X
内容記述
Astrocytes communicate with neurons through their processes. In vitro experiments have demonstrated that astrocytic processes exhibit calcium activity both spontaneously and in response to external stimuli; however, it has not been fully determined whether and how astrocytic subcellular domains respond to sensory input in vivo. We visualized the calcium signals in astrocytes in the primary visual cortex of awake, head‐fixed mice. Bias‐free analyses of two‐photon imaging data revealed that calcium activity prevailed in astrocytic subcellular domains, was coordinated with variable spot‐like patterns, and was dominantly spontaneous. Indeed, visual stimuli did not affect the frequency of calcium domain activity, but it increased the domain size, whereas tetrodotoxin reduced the sizes of spontaneous calcium domains and abolished their visual responses. The "evoked" domain activity exhibited no apparent orientation tuning and was distributed unevenly within the cell, constituting multiple active hotspots that were often also recruited in spontaneous activity. The hotspots existed dominantly in the somata and endfeet of astrocytes. Thus, the patterns of astrocytic calcium dynamics are intrinsically constrained and are subject to minor but significant modulation by sensory input.
本文を読む

http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/216672/1/phy2.12454.pdf

このアイテムのアクセス数:  回

その他の情報