Journal Article 13C/15N-Enriched l -Dopa as a Triple-Resonance NMR Probe to Monitor Neurotransmitter Dopamine in the Brain and Liver Extracts of Mice

Yamada, Hisatsugu  ,  Kameda, Tetsuro  ,  Kimura, Yu  ,  Imai, Hirohiko  ,  Matsuda, Tetsuya  ,  Sando, Shinsuke  ,  Toshimitsu, Akio  ,  Aoyama, Yasuhiro  ,  Kondo, Teruyuki

5 ( 2 )  , pp.125 - 128 , 2016-04-01 , Wiley-VCH Verlag
ISSN:2191-1363
Description
In an attempt to monitor μm-level trace constituents, we applied here 1H-{13C-15N} triple-resonance nuclear magnetic resonance (NMR) to 13C/15N-enriched l-Dopa as the inevitable precursor of the neurotransmitter dopamine in the brain. The perfect selectivity (to render endogenous components silent) and μm-level sensitivity (700 MHz spectrometer equipped with a cryogenic probe) of triple-resonance allowed the unambiguous and quantitative metabolic and pharmacokinetic analyses of administered l-Dopa/dopamine in the brain and liver of mice. The level of dopamine generated in the brain (within the range 7-76 μm, which covers the typical stimulated level of -30 μm) could be clearly monitored ex vivo, but was slightly short of the detection limit of a 7 T MR machine for small animals. This work suggests that μm-level trace constituents are potential targets of ex vivo monitoring as long as they contain N atom(s) and their appropriate 13C/15N-enrichment is synthetically accessible. Traces via triple resonance! Tripleresonance high-resolution NMR with a cryogenic probe at 16.4 T is sensitive and selective enough to perform quantitative metabolic/pharmacokinetic analysis of l-Dopa/dopamine in brain and liver extracts from mice. This work suggests that μm-level trace constituents are potential targets of ex vivo monitoring as long as they contain N atoms and their appropriate 13C/15N-enrichment is synthetically accessible.
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http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/216667/1/open.201500196.pdf

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