学術雑誌論文 Cryopreservation of the edible alkalophilic cyanobacterium Arthrospira platensis

Shiraishi, Hideaki

80pp.2051 - 2057 , 2016-05-31 , Taylor and Francis Group
ISSN:0916-8451
内容記述
Efficient cryopreservation conditions for the edible alkalophilic cyanobacterium Arthrospira (Spirulina) platensis were investigated using a model strain A. platensis NIES-39. As a result, it was found that more than 60% of cells were viable upon thawing, when they had been frozen at a cooling rate of approximately −1 °C min[−1] in the presence of 10% (v/v) dimethyl sulfoxide. Further examination with other Arthrospira strains showed that many of them had strain-dependent optimal conditions for cryopreservation. For example, the best freezing conditions for A. platensis SAG 21.99 were snap-freezing in liquid nitrogen in the presence of 5% (v/v) dimethyl sulfoxide, while they were slow cooling at approximately −1 °C min[−1] in the presence of 10% (v/v) methanol for A. platensis NIES-46, NIES-2308 and UTEX 1926. The variety of successful cryopreservation conditions presented in this study is useful when attempting to cryopreserve various Arthrospira strains.
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http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/216402/1/09168451.2016.1189320.pdf

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